Single Cell Isolation Microfluidics

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Single cell isolation microfluidics.

Methods currently used for single cell isolation include. 131 132 in parallel this technology has been developed commercially by gigagen. In this work a microfluidic method that is capable of isolating single cells in droplets selecting successful edited clones and expansion of these isoclones is introduced. Many single cell analysis techniques require the isolation of individual cells.

Here we present a microfluidic system that can overcome these limitations making use of a dual color signal normalization approach. Using a hybrid microfluidics method droplets in channels can be individually addressed using a co planar electrode system. Depending on the application single cell analysis chips can include any or all the above mentioned steps. 133 134 abvitro later acquired by juno therapeutics developed a similar approach for sequencing.

The throughput of this method was later increased to 10 6 cells by single cell isolation using droplet microfluidics instead of compartmentalization in microwells. We demonstrate the benefit of this approach to screen antibodies for the specific binding of cancer target cells in a high throughput single cell format which should have immense clinical potential.